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KMID : 0613820200300080688
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Journal of Life Science
2020 Volume.30 No. 8 p.688 ~ p.694
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The Protective Effects of an Ethyl Acetate Fraction of Raphiolepsis indica Against Oxidative Stress in HaCaT Keratinocytes
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Yang Eun-Ju
Kim Hye-Ran
Chang Kyung-Soo
Chang Jeong-Hyun
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Abstract
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In a previous study, the total phenolic content in ethanol extracts of medicinal plants that naturally grow on Jeju Island were analyzed with the extracts of Raphiolepsis indica leaf found to have the highest. The current study was carried out to evaluate the total flavonoid content, radical-scavenging activity, and the protective effect of R. indica extracts and solvent fractions on oxidative stress in HaCaT keratinocytes. More specifically, total flavonoid content and 2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging activity were measured to assess anti-oxidative activity, and protective effects against hydrogen peroxide (H2O2) were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay on the HaCaT cells. Of the various fractions analyzed, the ethyl acetate extract of R. indica showed the highest total flavonoid content (149.13 mg/g extracts) and the lowest remaining ABTS. In addition, the ethyl acetate fraction was significantly more resistant against H2O2 than the negative control. Our results therefore suggest that an ethyl acetate fraction of R. indica protects HaCaT cells against oxidative stress and could prove useful for developing functional cosmetic materials.
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KEYWORD
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Anti-oxidants, ethyl acetate fraction, keratinocytes, oxidative stress, Raphiolepis indica
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